Studies of the Rickettsial Plaque Assay Technique

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Studies of the rickettsial plaque assay technique.

A plaque assay system for pathogenic rickettsiae, which utilizes primary chick embryo tissue cultures, is described. It proved to be a highly reproducible measure of infectiousness for Rickettsia rickettsi and R. typhi, which were employed in most studies; as well as for R. canada, R. prowazeki, R. sibirica, R. akari, R. conori, and Coxiella burneti. Plaque-forming units (PFU) were compared to ...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1972

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.5.5.715-722.1972